“cytotoxicity Assay of Ethanolic Extracts of Amalakyadi Churna and Its Ingredients Using Artemia Salina”

Amalakyadi churna is an Ayurvedic formulation, which is extensively used in traditional system of Indian medicine and by folk practitioner for treating all types of fever, and other common ailments In this study, an equal concentration of ethanolic extracts of Amalakyadi churna and its ingredients (50μg/ml of artificial sea water) were subjected to a bioscreening study to detect the cytotoxic activity by the brine shrimp (Artemia salina) lethality bioassay. The results obtained from the crude ethanolic leaf extracts of Phyllanthus emblica L (73%), Terminalia chebula Retz (87%), Piper longum L (90%), Plumbago zeylanica L (93%), Amalakyadi churna (98%) were exhibited promising brine shrimp lethality. The present study supports that the brine shrimp bioassay is simple reliable and convenient method for assessment of bioactivity of these medicinal plants and lends support for their use in traditional medicine.